Composite

Part:BBa_M33354:Design

Designed by: Alejandro Virrueta   Group: Stanford BIOE44 - S010   (2010-06-06)


RBS+CDS for TodT protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 193
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made 1 silent mutation for XbaI, to ensure compliance with Assembly Standard 10. We also modified the stop codon to TAA, and added a strong ribosome binding site (BBa_B0034).


Source

This is a genomic sequence from Pseudomonas putida. The was obtained from GenBank at http://www.ncbi.nlm.nih.gov/nuccore/4914628 (Y18245.1; todT coordinates: 14162..14845).

References